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Imaging the awake visual cortex with a genetically encoded voltage indicator.

Matteo Carandini1, Daisuke Shimaoka2, L Federico Rossi3

  • 1UCL Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom, d.shimaoka@ucl.ac.uk m.carandini@ucl.ac.uk.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 9, 2015
PubMed
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This summary is machine-generated.

Genetically encoded voltage indicators (GEVIs) enable chronic, noninvasive imaging of neuronal membrane potential in awake mice. A new projection method effectively separates voltage signals from hemodynamic activity for clearer neural dynamics studies.

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Imaging

Background:

  • Genetically encoded voltage indicators (GEVIs) offer potential for noninvasive, chronic imaging of neuronal membrane potential.
  • Studying neural activity in awake animals is challenging due to confounding hemodynamic signals.

Purpose of the Study:

  • To test a GEVI (VSFP-Butterfly 1.2) in awake mouse cortex.
  • To develop a method for separating voltage signals from hemodynamic artifacts.
  • To compare GEVI performance with calcium imaging.

Main Methods:

  • In utero electroporation of VSFP-Butterfly 1.2 in mouse visual cortex.
  • Wide-field imaging using two cameras to capture dual fluorophore signals.
  • A projection method to differentiate voltage and hemodynamic signals.

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Main Results:

  • Successfully separated voltage and hemodynamic signals using weighted sums and differences.
  • Voltage signals exhibited fast dynamics consistent with neural activity and retinotopic mapping.
  • GEVI signals showed comparable signal-to-noise to calcium signals but were less susceptible to vascular artifacts.

Conclusions:

  • GEVIs are effective tools for studying neural population dynamics in the awake cortex at mesoscopic scales.
  • The developed projection method enhances the reliability of GEVI-based neural activity measurements.
  • GEVIs provide a valuable alternative to calcium imaging, particularly for subthreshold activity.