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Related Experiment Video

Updated: Apr 18, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

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Measuring absolute microvascular blood flow in cortex using visible-light optical coherence tomography.

Siyu Chen, Ji Yi, Sam Inayat

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |January 9, 2015
    PubMed
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    Phase sensitive Doppler optical coherence tomography measures absolute cerebral blood flow (CBF) in mouse brains. This technique provides detailed temporal and spatial CBF data and high-resolution microvasculature imaging.

    Area of Science:

    • Neuroscience
    • Biomedical Optics
    • Medical Imaging

    Background:

    • Cerebral blood flow (CBF) regulation is crucial for neurological health and disease research.
    • Accurate in vivo measurement of CBF is essential for understanding brain function and developing clinical diagnostics.

    Purpose of the Study:

    • To demonstrate the capability of phase sensitive Doppler optical coherence tomography (psD-OCT) for absolute CBF measurement in the mouse visual cortex.
    • To assess the accuracy and resolution of psD-OCT for analyzing regional CBF variations.
    • To visualize cortical microvasculature using enhanced contrast imaging.

    Main Methods:

    • Utilized phase sensitive Doppler optical coherence tomography operating in the visible-light spectral range.
    • Performed in vivo measurements of absolute CBF in the mouse visual cortex.

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    Last Updated: Apr 18, 2026

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  • Conducted in vitro experiments to validate measurement accuracy.
  • Enhanced blood vessel contrast to generate angiograms of cortical microvasculature.

    • Main Results:

    • Successfully measured absolute CBF in the mouse visual cortex in vivo.
    • Resolved both temporal and spatial profiles of regional CBF variations.
    • Demonstrated high consistency between in vitro measured and controlled CBF values.
    • Generated detailed angiograms of mouse cortical microvasculature.

    Conclusions:

    • Phase sensitive Doppler optical coherence tomography is a viable method for quantitative in vivo CBF measurement.
    • The technique offers high spatial and temporal resolution for studying CBF dynamics.
    • psD-OCT provides detailed visualization of cortical microvasculature, aiding in neurovascular research.