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Related Experiment Videos

Na+/H+ exchange and Ca2+ influx.

W Siffert1, J W Akkerman

  • 1Max-Planck-Institut für Biphysik, FRG.

FEBS Letters
|December 18, 1989
PubMed
Summary
This summary is machine-generated.

Cell stimulation increases intracellular calcium ([Ca2+]i) and activates sodium-hydrogen exchange, raising cytosolic pH (pHi). While pHi may regulate calcium channels, studies show calcium rises precede pHi increases, challenging alkalinization as the sole trigger.

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Area of Science:

  • Cellular Physiology
  • Ion Transport Mechanisms
  • Calcium Signaling

Background:

  • Cell stimulation elevates cytosolic free Ca2+ concentration ([Ca2+]i) and activates Na+/H+ exchange, leading to increased cytosolic pH (pHi).
  • The role of Na+/H+ exchange in regulating Ca2+ influx is under investigation, particularly whether elevated pHi alone can open plasma membrane Ca2+ channels.

Purpose of the Study:

  • To investigate the relationship between cytosolic pH (pHi) and Ca2+ influx.
  • To determine if cytosolic alkalinization is sufficient to trigger Ca2+ entry through plasma membrane channels.

Main Methods:

  • Utilized fluorescent indicators to measure simultaneous changes in cytosolic pH (pHi) and Ca2+ concentration ([Ca2+]i) in response to cell stimulation.
  • Examined the effects of artificial cytosolic alkalinization on Ca2+ uptake in various cell types.

Related Experiment Videos

  • Assessed the impact of inhibiting Na+/H+ exchange on agonist-induced Ca2+ influx.

    • Main Results:

    • Artificial cytosolic alkalinization induced Ca2+ uptake in endothelial cells, lymphocytes, and smooth muscle cells.
    • Inhibition of Na+/H+ exchange reduced agonist-induced Ca2+ influx in endothelial cells and platelets.
    • Crucially, measurements indicated that the rise in [Ca2+]i consistently preceded the rise in pHi, challenging the proposed causal link.

    Conclusions:

    • While Na+/H+ exchange and subsequent pHi changes appear to influence Ca2+ influx, the data challenges the notion that pHi elevation alone is sufficient to open Ca2+ channels.
    • The observed temporal sequence suggests that Ca2+ influx may initiate or occur concurrently with, rather than being solely triggered by, cytosolic alkalinization.