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Related Experiment Video

Updated: Apr 18, 2026

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

Ratiometric fluorescence imaging of cellular polarity: decrease in mitochondrial polarity in cancer cells.

Na Jiang¹, Jiangli Fan, Feng Xu

¹State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China).

Angewandte Chemie (International Ed. in English)

|January 10, 2015

Summary

A new fluorescent probe, BOB, enables ratiometric imaging of mitochondrial polarity. This probe can distinguish cancer cells from normal cells by detecting differences in mitochondrial polarity.

Keywords:

cancer sensing dyes mitochondria polarity ratiometric imaging

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Area of Science:

Biochemistry
Cell Biology
Molecular Imaging

Background:

Mitochondrial polarity is crucial for intracellular protein transport and biomacromolecular interactions.
Accurate measurement of mitochondrial polarity is essential for understanding cellular functions and disease states.

Purpose of the Study:

To develop the first fluorescent probe for ratiometric imaging of mitochondrial polarity.
To investigate the potential of using mitochondrial polarity differences to distinguish cancer cells from normal cells.

Main Methods:

Synthesis and characterization of a novel fluorescent probe, BOB, with dual absorption and emission maxima.
Evaluation of BOB's photophysical properties, including polarity sensitivity and spectral response.
Assessment of BOB's specificity for mitochondria and its independence from mitochondrial membrane potential.
Ratiometric fluorescence imaging of mitochondrial polarity in cancer and normal cells.

Main Results:

BOB exhibits dual absorption and emission spectra, with green emission sensitive to polarity changes.
The probe demonstrates a linear response across a wide range of solution polarities.
BOB shows high specificity for mitochondria (Rr =0.96), unaffected by mitochondrial membrane potential.
Mitochondrial polarity was found to be lower in cancer cells compared to normal cells.

Conclusions:

BOB is the first ratiometric fluorescent probe for mitochondrial polarity imaging.
The observed differences in mitochondrial polarity between cancer and normal cells offer a potential biomarker for cancer detection.
Ratiometric fluorescence imaging with BOB provides a novel approach for distinguishing cancer cells.