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Pedigree Analysis

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Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...

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Related Experiment Video

Updated: Apr 18, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

LINKPHASE3: an improved pedigree-based phasing algorithm robust to genotyping and map errors.

Tom Druet¹, Michel Georges¹

¹Unit of Animal Genomics, GIGA-R, University of Liège (B34), 1 avenue de l'Hôpital, B-4000, Liège, Belgium.

Bioinformatics (Oxford, England)

|January 10, 2015

Summary

This summary is machine-generated.

This study introduces a new phasing program for reconstructing genetic haplotypes in large half-sibling families. The program is robust to errors and more efficient than existing methods, especially with genotyping inaccuracies.

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Area of Science:

Genetics
Bioinformatics
Computational Biology

Background:

Haplotype reconstruction is crucial for genetic studies.
Existing methods face challenges with large families and data errors.

Purpose of the Study:

To develop a robust and efficient phasing program for large half-sibling families.
To improve haplotype reconstruction accuracy in the presence of genotyping and map errors.

Main Methods:

Developed a novel phasing program tailored for half-sibling pedigrees.
Evaluated program performance against existing methods using simulated and real data.
Assessed robustness against simulated genotyping and map errors.

Main Results:

The new program demonstrates superior efficiency compared to previous versions and other software.
The phasing program shows significant robustness to genotyping errors.
Effective for large half-sib families common in plant and animal genetics.

Conclusions:

The developed phasing program offers a more efficient and accurate solution for haplotype reconstruction in large half-sibling families.
This tool is particularly valuable for genetic applications dealing with imperfect genotype data.