Three-dimensional motion correction using speckle and phase for in vivo computed optical interferometric tomography

Area of Science:

• Optical Physics
• Tomography
• Image Processing

Background:

• Computed optical interferometric tomography enables high-resolution volumetric imaging.
• Interferometric techniques use phase and amplitude for aberration correction.
• Phase information increases sensitivity to motion, particularly along the optical axis.

Purpose of the Study:

• To develop algorithms for correcting 3D motion in computed optical interferometric tomography.
• To enable accurate post-acquisition correction of defocus and optical aberrations.
• To enhance the robustness of volumetric imaging techniques.

Main Methods:

• Algorithm 1: Utilizes phase differences in acquired data to correct axial motion.
• Algorithm 2: Employs a speckle tracking system with coherent illumination for orthogonal motion measurement.
• Coherent illumination ensures high-contrast speckle patterns for tracking.

Main Results:

• The combined algorithms effectively correct for motion in all three dimensions.
• Accurate correction allows for reliable defocus and aberration correction.
• The speckle tracking system functions even with uniform samples or aberrated beams.

Conclusions:

• The presented algorithms significantly improve the accuracy of computed optical interferometric tomography.
• 3D motion correction is crucial for reliable aberration and defocus correction.
• This work advances volumetric imaging by mitigating motion artifacts.