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Related Experiment Video

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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Polyvinylidene fluoride/silane-treated hydroxyapatite mixed matrix membrane for enzyme capturing.

Junfen Sun1, Zhenzhen Cao1, Lishun Wu2

  • 1State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, PR China.

Colloids and Surfaces. B, Biointerfaces
|January 10, 2015
PubMed
Summary

Modified nano-hydroxyapatite (HAP) particles with a silane coupling agent (KH792) created mixed matrix membranes (MMMs) for effective enzyme purification. These KH792-HAP embedded MMMs demonstrate superior distribution and adsorption capacity for enzyme capturing.

Keywords:
AdsorptionHAP modified with KH792 (KH792-HAP)Lysozyme (LZ)Mixed matrix membranes (MMMs)Polyvinylidene fluoride (PVDF)

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Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • Developing advanced materials for efficient enzyme purification is crucial in biotechnology.
  • Mixed matrix membranes (MMMs) offer potential for selective adsorption and separation processes.
  • Surface modification of nanoparticles can enhance their integration and performance within polymer matrices.

Purpose of the Study:

  • To modify nano-hydroxyapatite (HAP) particles using a silane coupling agent (KH792).
  • To prepare and characterize polyvinylidene fluoride (PVDF) based MMMs incorporating pure HAP and KH792-modified HAP (KH792-HAP).
  • To evaluate the enzyme adsorption capacity and purification potential of these MMMs using lysozyme (LZ) as a model enzyme.

Main Methods:

  • Surface modification of HAP nanoparticles with N-(β-aminoethyl)-γ-aminopropyltrimethoxy silane (KH792).
  • Fabrication of MMMs by embedding HAP and KH792-HAP into a PVDF matrix via immersion phase separation.
  • Characterization of MMMs using pure water flux, lysozyme (LZ) adsorption studies, and scanning electron microscopy (SEM).
  • Analysis of adsorption behavior using Langmuir and Freundlich isotherms.

Main Results:

  • KH792 modification improved the distribution of HAP within the PVDF matrix.
  • Langmuir adsorption isotherm models the adsorption behavior of both HAP and KH792-HAP more effectively than Freundlich.
  • The prepared MMMs demonstrated significant enzyme purification capabilities through both static and dynamic adsorption methods.
  • SEM analysis confirmed better dispersion of KH792-HAP in the polymer matrix.

Conclusions:

  • Surface modification of HAP with KH792 enhances its compatibility and dispersion within PVDF membranes.
  • The developed MMMs show promise as effective materials for enzyme capturing and purification.
  • These modified membranes offer a viable approach for enzyme purification techniques in biotechnological applications.