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Updated: Apr 18, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation of Sporothrix pallida complex in clinical and environmental samples from Chile]
Rodrigo M Cruz Choappa1, Peggy I Vieille Oyarzo2, Laura C Carvajal Silva3
1Cátedra de Micología, Facultad de Medicina, Universidad de Valparaíso, Valparaíso, Chile; Unidad de Infectología, Hospital Carlos van Buren de Valparaíso, Valparaíso, Chile.
Abstract:
The isolation of S. pallida complex from medical samples and home garden soil of a patient in Chile is here in reported. Fungi of the Sporothrix schenckii complex can cause various infections. In Chile, the medical and environmental isolates of these this complex are rare. The aim of this study was to identify an unusual agent in a case of onychomycosis and to detect its presence in the patient's home garden. For this purpose, clinical samples were obtained by scraping the patient's subungueal first right toe nail as well as by taking soil samples from different areas of her home garden. Species identification was performed by morphophysiology and one of the strains isolated from the patient's toe nail was sent to CBS for molecular confirmation (14.062). S. pallida complex was identified both from the patient's toe nail and samples taken from her home garden.

