Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing

Pre-mRNA Processing

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Benchmarking short- and long-read sequencing technologies for metagenomic profiling of microbiomes.

Scientific reports·2026

Same author

Federated, governed, and interoperable? The emerging architecture of public human genomic data infrastructures: a European perspective.

Frontiers in genetics·2026

Same author

Machine learning-based assessment of the healthy human gut mycobiota landscape using ITS1 DNA metabarcoding data.

BioData mining·2026

Same author

Enhanced Untargeted Metabolomics Based on High-Resolution Mass Spectrometry Reveals Global Rewiring Due to Mitochondrial Dysfunction in Yeast.

International journal of molecular sciences·2026

Same author

Single-cell transcriptomic profiling of human fetal neural stem cells isolated from the subventricular zone.

Frontiers in cell and developmental biology·2026

Same author

Editing-independent effects of <i>Drosophila</i> Adar on heterochromatin silencing.

RNA (New York, N.Y.)·2026

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Transcriptome assembly and alternative splicing analysis.

Paola Bonizzoni¹, Gianluca Della Vedova, Graziano Pesole

¹Department of Informatics, Systems and Communication (DISCo), University of Milano-Bicocca, Viale Sarca 336, Milano, 20126, Italy, bonizzoni@disco.unimib.it.

Methods in Molecular Biology (Clifton, N.J.)

|January 12, 2015

Summary

This summary is machine-generated.

Alternative Splicing (AS) prediction is crucial for understanding gene expression and disease. PIntron software accurately predicts gene exon-intron structures and transcript isoforms from genomic data.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Related Experiment Videos

Last Updated: Apr 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Alternative Splicing (AS) generates multiple transcripts from a single gene, expanding eukaryotic transcriptomes.
Aberrant AS is implicated in human disease development and progression.
Accurate characterization of gene exon-intron structures and transcript isoforms is biologically significant.

Purpose of the Study:

To introduce PIntron, a novel software package for predicting gene exon-intron structures and full-length transcript isoforms.
To provide an open-source, computationally efficient tool for AS analysis.

Main Methods:

PIntron utilizes genomic regions and transcript sets (ESTs/mRNAs) to predict exon-intron structures and isoforms.
The software is designed for standard workstations, handling large datasets efficiently.
Performance was evaluated on 112 well-annotated human genes from ENCODE regions.

Main Results:

PIntron achieves good accuracy in predicting exon-intron structures and transcript isoforms.
The software demonstrates efficiency in managing large genomic regions and extensive EST datasets.
Experimental evaluation confirmed the accuracy and usability of PIntron.

Conclusions:

PIntron offers a robust and accessible solution for computational prediction of alternative splicing events.
The software facilitates the characterization of gene structures and transcript repertoires.
PIntron is a valuable tool for researchers studying gene expression and AS-related diseases.