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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

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Updated: Apr 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

ASPicDB: a database web tool for alternative splicing analysis.

Mattia D'Antonio¹, Tiziana Castrgnanò, Matteo Pallocca

¹Consorzio Interuniversitario CINECA, 00185, Rome, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|January 12, 2015

Summary

This summary is machine-generated.

Alternative splicing (AS) generates diverse RNA molecules from single genes, impacting transcriptome complexity. The ASPicDB web application aids in studying these splicing events and their biological significance.

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Area of Science:

Molecular Biology
Bioinformatics
Genomics

Background:

Alternative splicing (AS) is a fundamental process in higher eukaryotes, generating transcriptomic and proteomic diversity from a single gene.
AS plays a crucial role in various biological processes and is implicated in diseases like cancer, with over 95% of human pre-mRNAs undergoing this modification.
Computational approaches and specialized databases are essential for understanding the complexity of AS.

Purpose of the Study:

To introduce ASPicDB, a novel web application designed for detailed analysis of the alternative splicing mechanism.
To provide powerful querying capabilities for investigating AS events across gene, transcript, and protein levels.
To offer integrated visualization tools for browsing and exporting AS data for further analysis.

Main Methods:

Development of the ASPicDB web application.
Implementation of querying systems for AS event interrogation.
Integration of web-based visualization tools for data exploration.

Main Results:

ASPicDB enables efficient recovery of detailed biological information regarding alternative splicing.
The application supports comprehensive analysis of AS at multiple biological levels.
Users can visualize and export splicing data for downstream investigations.

Conclusions:

ASPicDB serves as a valuable resource for researchers studying alternative splicing.
The tool facilitates a deeper understanding of AS mechanisms and their biological implications.
ASPicDB enhances the accessibility and analysis of complex splicing data.