Related Experiment Video
Updated: Apr 18, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Published on: August 21, 2016
Measuring helicase inhibition of the DEAD-box protein Dbp2 by Yra1
1Department of Biochemistry, Purdue University, BCHM 305, 175 S. University Street, West Lafayette, IN, 47907-2063, USA.
Abstract:
Despite the highly conserved helicase core, individual DEAD-box proteins are specialized in diverse RNA metabolic processes. One mechanism that determines DEAD-box protein specificity is enzymatic regulation by other protein cofactors. In this chapter, we describe a protocol for purifying the Saccharomyces cerevisiae DEAD-box RNA helicase Dbp2 and RNA-binding protein Yra1 and subsequent analysis of helicase regulation. The experiments described here can be adapted to other RNA helicases and their purified cofactor(s).
Related Concept Videos
Restarting Stalled Replication Forks
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle
DNA Helicases
Negative Regulator Molecules
Single-Strand DNA Binding Proteins

