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Development of Analytical Methods

Development of Analytical Methods

An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...

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Updated: Apr 18, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Green analytical method development for statin analysis.

Amira Louiza Assassi¹, Claude-Eric Roy¹, Philippe Perovitch²

¹Université de Bordeaux, EA 4575 Analytical and Pharmaceutical Developments Applied to Neglected Diseases and Counterfeits, Bordeaux, France.

Journal of Chromatography. A

|January 14, 2015

Summary

This summary is machine-generated.

A green analytical chemistry method using HPLC/DAD was developed for statin analysis. Optimized conditions achieved efficient separation and high solubility for potential buccal administration.

Keywords:

Ethanol Green analytical chemistry Solubility Statins Van Deemter

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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Area of Science:

Analytical Chemistry
Green Chemistry
Pharmaceutical Analysis

Background:

Development of efficient analytical methods for statins is crucial for pharmaceutical quality control.
Exploring green analytical chemistry principles reduces environmental impact.
High-concentration statin formulations require investigation for novel drug delivery systems.

Purpose of the Study:

To develop and validate a green High-Performance Liquid Chromatography with Diode-Array Detection (HPLC/DAD) method for analyzing pravastatin, fluvastatin, and atorvastatin.
To optimize column parameters and mobile phase composition for efficient statin separation.
To assess the solubility of statins in hydro-alcoholic solutions for potential buccal administration.

Main Methods:

HPLC/DAD analysis utilizing an ethanol-based mobile phase and an octadecyl-grafted silica column.
Optimization of column parameters (particle size, type) and mobile phase composition (ethanol/formic acid).
Validation of the method for content determination in high-concentration hydro-alcoholic solutions and assessment of statin solubility.

Main Results:

An ODS-AQ YMC column (50 mm × 4.6 mm, 3 μm) with an ethanol/formic acid mobile phase (50:50, v/v) at 1 mL/min and 40°C provided optimal separation and efficiency.
The highest concentration respecting detector linearity was 0.5 mg/mL for sample preparation.
Pravastatin and fluvastatin exhibited solubility >100 mg/mL, while atorvastatin calcium salt had a maximum solubility of 2 mg/mL in specific ethanol-water mixtures; using atorvastatin improved solubility.

Conclusions:

The developed green HPLC/DAD method is suitable for the content determination of statins in highly concentrated hydro-alcoholic solutions.
Optimized statin solutions demonstrate potential for buccal Per-Mucous administration due to rapid drug passage.
This research contributes to greener analytical practices and explores innovative drug delivery avenues for statins.