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A SepPak HPLC method for tricyclic antidepressant drugs in human vitreous humor
1University of Wisconsin, Department of Pathology, Madison 53706.
Journal of Analytical Toxicology
|November 1, 1989
Abstract:
Death from tricyclic antidepressant overdose has become an all-too-common occurrence. Several factors, including postmortem concentration changes, can render blood and tissue samples useless for the determination of antidepressant drug concentrations. We present here an efficient method of solid-phase extraction for these drugs from vitreous humor and a reversed-phase, isocratic high-performance liquid chromatographic method for the simultaneous quantitation of amitriptyline, doxepin, and imipramine and their desmethylated metabolites.