Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

25.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
25.1K
MicroRNAs01:22

MicroRNAs

4.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.4K
MicroRNAs01:22

MicroRNAs

12.1K
12.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.9K
3.9K
RNA Interference01:23

RNA Interference

29.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
29.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Diversity of Mouse dLGN Neurons and Modulation of Their Encoding Properties by Superior Colliculus.

Journal of integrative neuroscience·2026
Same author

Genomic sequence evolution underlying human neocortical interareal diversification.

Genome biology·2026
Same author

Parallel cholinergic circuit in oculomotor nucleus to control eye movements and REM sleep.

Nature communications·2026
Same author

Dopamine inhibits retinal pathological neovascularization in the oxygen-induced retinopathy mouse model.

The Journal of pathology·2026
Same author

Genetic and epidemiological analyses of alcohol consumption patterns and age-related macular degeneration risk among current drinkers: a role for TNFRSF10A.

Journal of global health·2026
Same author

Functional targeting of PTTG1 in pericytes restores vascular integrity in diabetic retina.

Science advances·2026

Related Experiment Video

Updated: Apr 18, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

8.0K

lncRNA-MIAT regulates microvascular dysfunction by functioning as a competing endogenous RNA.

Biao Yan1, Jin Yao2, Jing-Yu Liu2

  • 1From the Department of Central Laboratory, Eye Hospital (B.Y., J.-Y.L., J.Y., X.-M.L., X.-Q.W., Y.-J.L., Z.-F.T., Y.-C.S., Q.J.), Department of Ophthalmology, The Fourth School of Clinical Medicine (B.Y., J.Y., Q.J.), and Department of Pathophysiology, School of Basic Medical Sciences (Q.C.), Nanjing Medical University, Nanjing, China. yanbiao1982@hotmail.com jqin710@vip.sina.com.cn.

Circulation Research
|January 15, 2015
PubMed
Summary

Long noncoding RNA MIAT is implicated in diabetes-induced microvascular dysfunction. Targeting MIAT may offer new therapeutic strategies for neovascular diseases by regulating pathological angiogenesis.

Keywords:
angiogenesis inhibitorsepigenomicslong noncoding RNAvascular endothelial growth factor

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K

Related Experiment Videos

Last Updated: Apr 18, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

8.0K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Ophthalmology

Background:

  • Pathological angiogenesis drives diseases like cancer and ocular disorders.
  • Long noncoding RNAs (lncRNAs) regulate key biological processes involved in angiogenesis.
  • The role of lncRNAs in diabetes mellitus-induced microvascular dysfunction remains unclear.

Purpose of the Study:

  • To investigate the involvement of lncRNA-MIAT in diabetes mellitus-induced microvascular dysfunction.
  • To understand the regulatory mechanism of MIAT in diabetic retinopathy.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) to measure MIAT expression.
  • In vivo studies using diabetic mouse models and in vitro endothelial cell assays.
  • Bioinformatics analysis, luciferase assays, and RNA immunoprecipitation to elucidate molecular interactions.

Main Results:

  • Increased expression of lncRNA-MIAT was observed in diabetic retinas and high glucose-treated endothelial cells.
  • MIAT knockdown ameliorated diabetic microvascular dysfunction and inhibited endothelial cell proliferation, migration, and tube formation.
  • MIAT acts as a competing endogenous RNA, forming a feedback loop with VEGF and miR-150-5p.

Conclusions:

  • lncRNA-MIAT plays a significant role in pathological angiogenesis in diabetes.
  • MIAT is a potential diagnostic and therapeutic target for neovascular diseases.