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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
30.3K
Directing parthenogenetic stem cells differentiate into adipocytes for engineering injectable adipose tissue
Wei Liu1, Xingyuan Yang2, Xingrong Yan1
1Rege Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 Taibai Road, Xi'an 710032, China.
Stem Cells International
|January 15, 2015
Summary
Parthenogenetic embryonic stem cells (pESCs) can be induced to become adipogenic cells for engineering injectable adipose tissue. This study demonstrates pESCs
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Tissue Engineering
Background:
- Adipose tissue engineering requires suitable seed cells.
- Parthenogenetic embryonic stem cells (pESCs) possess pluripotent differentiation capacity.
Purpose of the Study:
- To stepwise induce pESCs into adipogenic cells.
- To engineer injectable adipose tissue using these cells.
Main Methods:
- Stepwise induction of pESCs to adipogenic cells.
- Isolation and expansion of embryonic mesenchymal stem cells (eMSCs) from embryoid bodies.
- Subcutaneous injection of engineered adipose tissue constructs in nude mice.
Main Results:
- eMSCs exhibited characteristics similar to bone marrow mesenchymal stem cells (BMSCs).
- Induced adipogenic cells showed increased adipose-specific gene expression and lipid accumulation.
- Successful fabrication of new adipose tissue in vivo using pESC-derived cells.
Conclusions:
- pESCs are a promising cell source for adipose tissue engineering.
- Engineered injectable adipose tissue can be fabricated from pESC-derived adipogenic cells.
