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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
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Structural characterisation of a polysaccharide from radix ranunculus ternati
Xuefeng Huang1, Yun Zhao2, Xin Jin2
1Zhongshan Hospital, Xiamen University, Xiamen 361000, China .
Iranian Journal of Pharmaceutical Research : IJPR
|January 15, 2015
Summary
Researchers isolated a water-soluble polysaccharide, HB-1, from radix Ranunculi ternati. This complex carbohydrate showed no inhibition activity against human glioma cells.
Area of Science:
- Carbohydrate Chemistry
- Natural Product Chemistry
- Pharmacology
Background:
- Radix Ranunculi ternati is a traditional medicinal herb.
- Polysaccharides are known for diverse biological activities.
- Characterization of plant-derived polysaccharides is crucial for understanding their potential applications.
Purpose of the Study:
- To isolate and characterize a novel water-soluble polysaccharide (HB-1) from Radix Ranunculi ternati.
- To determine the structural features of HB-1, including sugar composition and linkage patterns.
- To evaluate the in vitro inhibitory activity of HB-1 against a human glioma cell line.
Main Methods:
- Hot water extraction and ethanol precipitation for polysaccharide isolation.
- Deproteination, ultrafiltration, and gel-filtration column chromatography for purification.
- Gas-liquid chromatography (GLC) for sugar composition analysis.
- Smith degradation and methylation analysis for structural elucidation.
Main Results:
- A water-soluble polysaccharide, HB-1, with a molecular weight of 23,930 Da was successfully isolated.
- HB-1 comprised glucose (Glc), arabinose (Ara), and galactose (Gal) in a molar ratio of approximately 16.1:2.7:1.
- Structural analysis indicated a complex arrangement of glycosidic linkages, with a proposed repetitive unit structure.
Conclusions:
- The polysaccharide HB-1 from Radix Ranunculi ternati possesses a defined structure with a high glucose content.
- HB-1 demonstrated no significant inhibitory activity against the SF188 human glioma cell line in vitro.
- Further research is needed to explore other potential biological activities of HB-1 or related compounds.
Keywords:
Absolute configurationAnti-tumor activityMethylation reactionPolysaccharideRanunculus ternatus ThunbSmith degradationUltrafiltrationMore Related Videos
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