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Updated: Apr 18, 2026
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
A phage display screening derived Peptide with affinity for the adeninyl moiety
Louise Elmlund1, Pernilla Söderberg1, Subramanian Suriyanarayanan1
1Bioorganic & Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry, Linnæus University, SE-39182 Kalmar, Sweden; E-Mails: louise.elmlund@lnu.se (L.E.); pernilla.soderberg@lnu.se (P.S.); esusu@lnu.se (S.S.) ; Department of Chemistry & Biomedical Sciences, Linnæus University, SE-39182 Kalmar, Sweden.
Abstract:
Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (K D of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions.
