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Updated: Apr 18, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication
Luana de Borba1, Sergio M Villordo1, Nestor G Iglesias1
1Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
Abstract:
The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.
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