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08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
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Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication
Luana de Borba1, Sergio M Villordo1, Nestor G Iglesias1
1Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
Journal of Virology
|January 16, 2015
Summary
New dengue virus RNA elements in the capsid gene enhance viral replication. This interaction between the capsid and 3' untranslated region (UTR) differs between mosquito and mammalian cells.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- The dengue virus genome is a dynamic RNA molecule.
- Viral RNA structure plays a critical role in replication.
- Understanding RNA conformational changes is key to controlling viral infection.
Purpose of the Study:
- To identify novel cis-acting elements within the dengue virus genome.
- To investigate the role of RNA-RNA interactions in viral replication.
- To understand differential replication mechanisms in various hosts.
Main Methods:
- RNA folding predictions
- Chemical probing analysis
- RNA binding assays
- Functional studies of viral replication
Main Results:
- Identified new cis-acting elements in the capsid coding sequence.
- Demonstrated facilitation of viral RNA cyclization via hybridization.
- Showed differential enhancement of viral replication in mosquito and mammalian cells.
Conclusions:
- A novel RNA-RNA interaction involving the capsid coding sequence and the 3' UTR influences dengue virus replication.
- This interaction differentially impacts viral replication efficiency in distinct cellular environments.
- Findings provide insights into host-specific viral dynamics and potential therapeutic targets.
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