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In vitro aggregation assays using hyperphosphorylated tau protein.

Dexin Sui¹, Mengyu Liu¹, Min-Hao Kuo²

¹Department of Biochemistry and Molecular Biology, Michigan State University.

Journal of Visualized Experiments : Jove

|January 16, 2015

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Researchers developed new assays using hyperphosphorylated tau to find Alzheimer's disease and tauopathy therapeutics. These high-throughput screens can identify compounds targeting abnormal tau protein aggregation.

Area of Science:

Neuroscience
Biochemistry
Pharmacology

Background:

Alzheimer's disease and other tauopathies involve neurofibrillary tangles (NFTs) formed by hyperphosphorylated tau protein.
NFT density correlates with cognitive decline, highlighting the need for tau-targeting therapies.
Current tau aggregation assays lack pathology-relevant phosphorylation, limiting therapeutic discovery.

Purpose of the Study:

To develop novel assays for studying hyperphosphorylated tau aggregation.
To enable high-throughput screening for compounds modulating hyperphosphorylated tau kinetics and stability.

Main Methods:

Development of two assays utilizing recombinant, hyperphosphorylated tau.
Adaptation of assays for scalability in high-throughput screening.

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In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

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Published on: January 2, 2015

Main Results:

Successful establishment of assays using hyperphosphorylated tau.
Demonstrated potential for screening compounds affecting tau aggregate stability and kinetics.

Conclusions:

These assays provide a valuable tool for discovering novel therapeutics for Alzheimer's disease and tauopathies.
Utilizing hyperphosphorylated tau isoforms in assays is crucial for identifying effective treatments.