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Related Experiment Videos

Double Ds elements are involved in specific chromosome breakage.

H P Döring1, B Nelsen-Salz, R Garber

  • 1Institut für Genetik, Universität zu Köln, Federal Republic of Germany.

Molecular & General Genetics : MGG
|October 1, 1989
PubMed
Summary

Double Dissociation (Ds) elements in maize can form symmetrical structures that lead to chromosome breakage. These structures, when excised, leave behind half Ds elements, causing genomic deletions and altered breakage patterns.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • The maize sucrose synthase gene (Sh) is crucial for plant development.
  • Unstable alleles, like sh-m5933, are often associated with transposable elements.
  • Dissociation (Ds) elements are known to cause genetic instability in maize.

Purpose of the Study:

  • To analyze the structure of the Ds-induced sh-m5933 allele in maize.
  • To investigate the mechanism of Ds-induced chromosome breakage.
  • To understand the role of double Ds structures in genomic rearrangements.

Main Methods:

  • DNA structural analysis of the sh-m5933 allele.
  • Identification and characterization of Ds elements and flanking DNA.
  • Analysis of revertant strains with altered chromosome breakage patterns.

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  • Genomic DNA deletion analysis.

    • Main Results:

    • A symmetrical double Ds structure was identified flanking a 30 kb insert in the sucrose synthase gene.
    • Excising one Ds element left a half Ds element, leading to altered chromosome breakage.
    • Nine new maize strains exhibited similar deletions, suggesting a common excision mechanism.
    • Double Ds structures were confirmed as the cause of Ds-induced chromosome breakage.

    Conclusions:

    • Symmetrical double Ds structures are responsible for Ds-induced chromosome breakage in maize.
    • Half Ds elements can result from Ds excision and lead to genomic instability.
    • The findings provide insights into the mechanisms of transposable element-mediated rearrangements.