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Published on: February 27, 2014
Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression
Sehe Dong Lee1, Jung Wan Choe1, Beom Jae Lee1
1Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
Aim:
To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10(-/-) mice.
Methods:
To synchronize colitis, 8- to 10-wk-old IL-10(-/-) mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10(-/-) mice and in Colo 205 cells.
Results:
Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the butein-treated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells.
Conclusion:
Butein ameliorated colitis in IL-10(-/-) mice by regulating IL-6/STAT3 and MMP-9 activation.
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