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Autophagy

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...

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Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

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Updated: Apr 18, 2026

Study of Protein-protein Interactions in Autophagy Research

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Methods to study chaperone-mediated autophagy.

Bindi Patel¹, Ana Maria Cuervo¹

¹Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Methods (San Diego, Calif.)

|January 18, 2015

Summary

This summary is machine-generated.

Chaperone-mediated autophagy (CMA) degrades cytosolic proteins in lysosomes. This study details methods to monitor CMA, a process crucial for proteome maintenance and implicated in diseases like neurodegeneration and cancer.

Keywords:

Autophagy Fluorescent reporters Lysosomes Organelle isolation Proteolysis

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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

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Published on: July 27, 2018

Area of Science:

Cell Biology
Molecular Biology
Autophagy Research

Background:

Chaperone-mediated autophagy (CMA) is a key pathway for degrading soluble cytosolic proteins.
CMA maintains proteome stability and recycles amino acids, impacting cellular energy.
Dysfunctional CMA is linked to neurodegeneration, cancer, immunodeficiency, and diabetes.

Purpose of the Study:

To describe methods for studying chaperone-mediated autophagy (CMA).
To provide tools for monitoring CMA in various experimental settings.
To advance understanding of CMA's role in health and disease.

Main Methods:

Selective targeting of cytosolic substrates to lysosomes via chaperones.
Translocation of substrates into the lysosome through a specific receptor complex.
Utilizing diverse experimental models to investigate CMA processes.

Main Results:

The abstract does not contain specific results, but outlines the described approaches.
The described approaches allow for the study of CMA substrate recognition and translocation.
Methods enable monitoring of CMA activity in different cellular contexts.

Conclusions:

Understanding CMA is critical due to its role in cellular homeostasis.
Defects in CMA are associated with significant human pathologies.
The described methods facilitate research into CMA and its associated diseases.