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Patterning of Embryonic Stem Cells Using the Bio Flip Chip
Published on: October 1, 2007
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nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip.
S Menad1, L Franqueville1, N Haddour1
1Université de Lyon, Ecole centrale de Lyon, CNRS UMR 5005, Laboratoire Ampère, 69130 Ecully, France.
Acta Biomaterialia
|January 18, 2015
Summary
This study introduces a novel bioelectronic chip for cell assembly using negative dielectrophoresis (nDEP). This method enables controlled formation of cell aggregates and patterned cell arrays for tissue engineering and cell interaction studies.
Area of Science:
- Biotechnology
- Bioengineering
- Cell Biology
Background:
- Controlled cell assembly is crucial for tissue engineering and studying cell interactions.
- Existing bottom-up approaches face challenges in creating precise cell aggregates and patterns.
Purpose of the Study:
- To develop and demonstrate a new bioelectronic chip for cell assembly using negative dielectrophoresis (nDEP).
- To enable the formation of both compact cell aggregates and patterned cell arrays on substrates.
Main Methods:
- Utilized a bioelectronic chip with a quadripolar electrode array and a micropatterned PDMS membrane.
- Employed negative dielectrophoresis (nDEP) for cell manipulation and assembly.
- Investigated cell viability and spreading activity post-assembly.
Main Results:
- Successfully assembled cells into compact aggregates and onto defined areas using the nDEP chip.
- Demonstrated the formation of detachable cell aggregates by leveraging cell-cell adhesion.
- Confirmed cell viability and assessed cell spreading post-nDEP assembly.
Conclusions:
- The developed bioelectronic chip effectively facilitates nDEP-driven cell assembly for tissue engineering.
- This technique offers a versatile platform for creating cellular structures and studying cell behavior.
