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Protein behavior at surfaces: orientation, conformational transitions and transport
Linling Yu1, Lin Zhang1, Yan Sun1
1Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Journal of Chromatography. A
|January 21, 2015
Summary
This study overviews protein behavior in chromatography, focusing on surface interactions, unfolding, and refolding. Advances in molecular simulations and experiments inform the design of high-performance protein chromatography materials and conditions.
Area of Science:
- Biochemistry and Biophysics
- Chemical Engineering
- Materials Science
Background:
- Chromatography is fundamental for protein purification and refolding.
- Understanding protein-surface interactions is crucial for optimizing chromatographic processes.
Purpose of the Study:
- To review scientific and technological advancements in protein chromatography.
- To explore protein behavior at chromatographic surfaces, including orientation, conformational changes, and transport.
- To highlight implications for designing advanced protein chromatography systems.
Main Methods:
- Review of recent scientific literature.
- Analysis of molecular simulations.
- Integration of theoretical and experimental investigations.
Main Results:
- Detailed examination of protein orientation and conformational transitions (unfolding/refolding) on surfaces.
- Discussion of protein transport phenomena in chromatographic systems.
- Identification of key factors influencing protein behavior.
Conclusions:
- Molecular simulations and experimental data offer insights into protein-surface interactions.
- These findings guide the rational design of novel chromatographic materials and mobile phase conditions.
- Advancements aim to enhance the performance of protein chromatography for purification and refolding.
Keywords:
Conformational transitionMass transportOrientationProtein chromatographySurface behaviorSurface diffusionMore Related Videos
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