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Updated: Apr 18, 2026
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
Published on: May 5, 2020
let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans
Maha Z Alriyami1, Martin R Jones2, Robert C Johnsen3
1Department of Molecular Biology and Biochemistry, Simon Fraser University Burnaby, 8888 University Drive, Burnaby, BC V5A 1S6 Canada ; Department of Biochemistry, College of Medicine and Health Sciences, Sultan Qaboos University, 35, Al-Khod 123, Oman.
Abstract:
Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.
