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Related Experiment Video

Updated: Apr 18, 2026

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Lasing from fluorescent protein crystals.

Heon Jeong Oh, Malte C Gather, Ji-Joon Song

|January 22, 2015

Summary

Researchers created fluorescent protein crystals for photonic applications. These protein crystals show enhanced fluorescence and can generate laser emission, offering potential for novel micro-lasers.

Area of Science:

Biophysics
Materials Science
Photonics

Background:

Fluorescent proteins are widely used in biological imaging.
Ordered protein structures offer unique optical properties not seen in solution.

Purpose of the Study:

To investigate fluorescent protein crystals for photonic applications.
To explore the optical properties and potential lasing capabilities of synthesized enhanced green fluorescent protein (EGFP) crystals.

Main Methods:

Synthesis of rod-shaped enhanced green fluorescent protein (EGFP) crystals.
Characterization of crystal morphology and optical properties.
Measurement of fluorescence intensity and concentration quenching.
Optical pumping experiments to induce laser emission.

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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Related Experiment Videos

Last Updated: Apr 18, 2026

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Main Results:

Synthesized EGFP crystals with dimensions of 0.5-2 μm in diameter and 100-200 μm in length.
Observed minimal light scattering and significantly higher fluorescence intensity compared to EGFP in solution.
Quantified concentration quenching in EGFP crystals at 7-10 dB.
Achieved single-mode laser emission at 513 nm upon optical pumping at 485 nm.

Conclusions:

Protein crystals are promising novel optical elements.
Demonstrated potential for self-assembled micro- or nano-lasers and amplifiers in aqueous environments.
Highlights the utility of ordered protein structures for advanced photonic devices.