Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

10.4K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
10.4K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.5K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.5K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.4K
COP Coated Vesicles00:59

COP Coated Vesicles

18.8K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.8K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

11.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
11.5K
Transport Across the Golgi01:26

Transport Across the Golgi

6.8K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clicking 1,4-Dithiin Conjugated Dimaleimides for Chiroptical Evolution and Nanofabrication.

Nano letters·2026
Same author

Chalcogen-bonding-mediated chiral recognition and enantioenrichment of organoselenocyanates.

Chemical science·2026
Same author

π-low transition temperature mixtures with widely-tunable polarity and ultralow viscosity for synthesizing chiral nanomaterials.

Nature communications·2026
Same author

Solvent-Gated Multimodal Charge Transfer Interactions for Precise Supramolecular Chirality Control.

Angewandte Chemie (International ed. in English)·2026
Same author

Click/release reaction and SO<sub>2</sub> recycling for multichannel dynamic chiroptical materials.

Nature communications·2026
Same author

Chiral inheritance effect in the reactive cystine-based coassembly system.

Nature communications·2026

Related Experiment Video

Updated: Apr 18, 2026

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K

An easy approach for constructing vesicles by using aromatic molecules with β-cyclodextrin.

Shangyang Li1, Lin Zhang, Bo Wang

  • 1School of Chemistry and Chemical Engineering and Key Laboratory of Colloid and Interface Chemistry of Ministry of Education, Shandong University, Jinan 250100, PR China. haoay@sdu.edu.cn.

Soft Matter
|January 22, 2015
PubMed
Summary

Researchers developed simple, stimuli-responsive vesicles using beta-cyclodextrin (β-CD) and aromatic molecules. This novel self-assembly strategy offers new possibilities for creating advanced nanocarriers and soft materials.

More Related Videos

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.5K
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
05:43

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Published on: January 24, 2017

15.3K

Related Experiment Videos

Last Updated: Apr 18, 2026

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.5K
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
05:43

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Published on: January 24, 2017

15.3K

Area of Science:

  • Supramolecular chemistry
  • Materials science
  • Nanotechnology

Background:

  • Vesicles are crucial nanostructures for drug delivery and materials science.
  • Developing simple and versatile methods for vesicle formation is an ongoing challenge.
  • Beta-cyclodextrin (β-CD) complexes offer potential for novel self-assembly systems.

Purpose of the Study:

  • To develop an easy and efficient method for preparing vesicles using β-cyclodextrin (β-CD) complexes.
  • To characterize the structure and self-assembly mechanism of these novel vesicles.
  • To explore the stimuli-responsive properties of the prepared vesicular architectures.

Main Methods:

  • Vesicle formation using β-cyclodextrin (β-CD) and ultra-small aromatic molecules in aqueous solution.
  • Structural characterization via transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic laser light scattering (DLS).
  • Investigation of self-assembly factors using proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).

Main Results:

  • Successfully formed stable vesicles through a straightforward self-assembly process.
  • Identified key structural factors driving self-assembly in the β-CD/l-phenylalanine system.
  • Demonstrated tunable stimuli-responsive behavior by incorporating various guest molecules.

Conclusions:

  • A novel and uncomplicated strategy for synthesizing stimuli-responsive vesicles using β-CD complexes has been established.
  • The findings provide fundamental insights into the self-assembly mechanisms of β-CD-based supramolecular systems.
  • This approach opens new avenues for the design and application of advanced nanocarriers and soft materials.