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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Apr 18, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
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A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

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Collaborative computational project for electron cryo-microscopy.

Chris Wood1, Tom Burnley1, Ardan Patwardhan2

  • 1Scientific Computing Department, Science and Technology Facilities Council, Research Complex at Harwell, Didcot OX11 0FA, England.

Acta Crystallographica. Section D, Biological Crystallography
|January 24, 2015
PubMed
Summary
This summary is machine-generated.

The new Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM) aims to unite scientists and improve cryo-electron microscopy software. This initiative will foster a supportive community and enhance software development for better research outcomes.

Keywords:
CCP-EMCollaborative Computational Project for Electron cryo-Microscopy

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Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Electron cryo-microscopy (cryoEM) is a powerful technique for determining molecular structures.
  • There is a need for a dedicated computational project to support the growing cryoEM community and software development.
  • Existing computational projects like CCP4 for crystallography provide a successful model.

Purpose of the Study:

  • To establish a collaborative computational project for cryoEM (CCP-EM).
  • To build a cohesive cryoEM community offering support to scientists.
  • To enhance the development and dissemination of robust and user-friendly cryoEM software.

Main Methods:

  • Leveraging common interests with CCP4, such as model fitting and software libraries.
  • Developing and disseminating robust and user-friendly cryoEM software.
  • Fostering community engagement and support for practicing scientists and software developers.

Main Results:

  • The establishment of the Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM).
  • Progress in building a coherent cryoEM community and providing support.
  • Exploitation of common areas with CCP4 for software development.

Conclusions:

  • CCP-EM is crucial for advancing cryoEM research by supporting scientists and developers.
  • The project aims to create a centralized hub for cryoEM computational resources and expertise.
  • Future plans involve continued development and community engagement to address the diverse needs of cryoEM techniques.