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Updated: Apr 18, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Chemical genomic profiling via barcode sequencing to predict compound mode of action
Jeff S Piotrowski1, Scott W Simpkins, Sheena C Li
1Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WEI4152, 1552 University Ave., Madison, WI, 53726, USA, jpiotrowski@wisc.edu.
Abstract:
Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.
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