Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA damage in macrophages drives immune autoreactivity via nuclear antigen presentation.

Nature aging·2026
Same author

A Simple Nonviral Method to Generate Human Induced Pluripotent Stem Cells Using SMAR DNA Vectors.

Genes·2024
Same author

Development of an Orthotopic HPV16-Dependent Base of Tongue Tumor Model in MHC-Humanized Mice.

Pathogens (Basel, Switzerland)·2023
Same author

An episomal DNA vector platform for the persistent genetic modification of pluripotent stem cells and their differentiated progeny.

Stem cell reports·2021
Same author

Safe and stable generation of induced pluripotent stem cells using doggybone DNA vectors.

Molecular therapy. Methods & clinical development·2021
Same author

Peptide Mini-Vectors for Gene Delivery.

Angewandte Chemie (International ed. in English)·2021

Related Experiment Video

Updated: Apr 18, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

38.2K

Sustained expression from DNA vectors.

Suet Ping Wong1, Orestis Argyros2, Richard P Harbottle3

  • 1Leukocyte Biology Section, National Heart & Lung Institute, Imperial College London, London, UK.

Advances in Genetics
|January 27, 2015
PubMed
Summary
This summary is machine-generated.

Developing effective DNA vectors for gene therapy requires overcoming the body's natural defenses against foreign DNA. Strategies are needed to ensure sustained therapeutic gene expression for successful clinical applications.

Keywords:
DNA vectorsEpisomal maintenanceGene silencingLong-term transgene expressionMinicirclesMitotic stabilityNonintegrating vectorsNonviral vectorsS/MARTransfectionVector loss

More Related Videos

Production of Double-stranded DNA Ministrings
06:12

Production of Double-stranded DNA Ministrings

Published on: February 29, 2016

10.8K
Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

10.0K

Related Experiment Videos

Last Updated: Apr 18, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

38.2K
Production of Double-stranded DNA Ministrings
06:12

Production of Double-stranded DNA Ministrings

Published on: February 29, 2016

10.8K
Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

10.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • The human body possesses defense mechanisms to detect and silence foreign DNA, often encountered during infection or DNA damage.
  • Non-viral DNA vectors frequently trigger these defenses, leading to their degradation or silencing, limiting therapeutic efficacy.
  • Transient transgene expression from unmodified DNA vectors restricts their clinical utility in gene therapy.

Purpose of the Study:

  • To elucidate the mechanisms by which the human body destroys or silences DNA vectors.
  • To explore strategies for enhancing the persistence and expression levels of therapeutic transgenes delivered by DNA vectors.
  • To address a key challenge in non-viral gene therapy research: the development of safe and persistently expressing DNA vectors.

Main Methods:

  • Review of current understanding of DNA vector degradation and silencing pathways.
  • Analysis of various strategies employed to improve DNA vector stability and transgene expression.
  • Discussion of mechanisms to evade host immune responses and cellular defenses.

Main Results:

  • Identification of host defense mechanisms as a primary barrier to effective gene therapy.
  • Demonstration that many non-viral vectors are susceptible to rapid clearance or silencing.
  • Highlighting the necessity of vector design modifications to achieve sustained therapeutic effects.

Conclusions:

  • Overcoming innate cellular defenses is critical for the clinical success of DNA vector-based gene therapy.
  • Strategic vector design is essential to ensure long-term, stable transgene expression.
  • Further research into vector-host interactions is crucial for advancing non-viral gene therapy.