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Updated: Apr 18, 2026

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Highly Sensitive DNA Sensor Based on Upconversion Nanoparticles and Graphene Oxide.

P Alonso-Cristobal1, P Vilela2, A El-Sagheer3

  • 1†Department of Physical Chemistry II, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain.

ACS Applied Materials & Interfaces
|January 28, 2015
PubMed
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This summary is machine-generated.

This study presents a novel DNA biosensor utilizing fluorescence resonance energy transfer (FRET) between specialized nanoparticles and graphene oxide. The sensor achieves highly sensitive and specific DNA detection down to 5 picomolar concentrations.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Fluorescence resonance energy transfer (FRET) is a powerful tool for studying molecular interactions.
  • Graphene oxide (GO) possesses unique optical properties making it suitable for sensing applications.
  • Developing sensitive and specific DNA detection methods is crucial for diagnostics and research.

Purpose of the Study:

  • To develop a novel DNA biosensor using FRET between NaYF4:Yb,Er nanoparticles and graphene oxide.
  • To investigate the mechanism of FRET-based DNA detection mediated by π-π stacking interactions.
  • To evaluate the sensitivity and specificity of the developed DNA biosensor.

Main Methods:

  • Synthesis of monodisperse NaYF4:Yb,Er nanoparticles coated with a SiO2 shell.
Keywords:
DNA biosensorFRETgraphene oxideupconversion nanoparticles

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  • Functionalization of nanoparticles with single-stranded DNA.
  • Utilizing the FRET mechanism between DNA-functionalized nanoparticles and graphene oxide for detection.
  • Assessing sensor performance through fluorescence quenching and hybridization studies.

    • Main Results:

    • Demonstrated FRET-based fluorescence quenching of NaYF4:Yb,Er@SiO2 nanoparticles by graphene oxide due to DNA-GO interaction.
    • Observed recovery of fluorescence upon hybridization with complementary DNA, indicating specific detection.
    • Achieved a low detection limit of 5 picomolar for DNA detection.
    • Exhibited high sensitivity and specificity in DNA detection.

    Conclusions:

    • The developed FRET-based DNA biosensor offers a sensitive and specific method for DNA detection.
    • The sensor leverages the interaction between DNA and graphene oxide surfaces for signal transduction.
    • This approach provides a new avenue for developing advanced biosensing platforms.