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FcUni-RLuc: an engineered Renilla luciferase with Fc binding ability and light emission activity
A Farzannia1, R Roghanian, S H Zarkesh-Esfahani
1Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
The Analyst
|January 28, 2015
Abstract:
A novel and advanced Fc-binding probe – FcUni-RLuc namely – has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence – HWRGWV – was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.

