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Ara-C increases gastric cancer cell invasion by upregulating CD-147-MMP-2/MMP‑9 via the ERK signaling pathway

Guang-Lin Yang1, Hai-Rong Tao2, Han-Wei Wang3

  • 1Department of General Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.

Oncology Reports
|January 28, 2015
PubMed

Insights

Low-dose Ara-C increases gastric cancer cell invasion by upregulating CD-147, MMP-2, and MMP-9 via the ERK pathway. This finding helps prevent chemotherapy side effects.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Gastric cancer cells show limited sensitivity to Ara-C, a DNA synthesis inhibitor.
  • Understanding Ara-C's effects on gastric cancer cell proliferation and invasion is crucial.

Purpose of the Study:

  • To investigate the role of Ara-C in gastric cancer cell proliferation, apoptosis, and invasion.
  • To elucidate the molecular mechanisms underlying Ara-C's effects on gastric cancer cells.

Main Methods:

  • Cell Counting Kit-8 for proliferation assessment.
  • Gelatin zymography for MMP-2 and MMP-9 activity.
  • RT-PCR and Western blotting for gene and protein expression analysis (CD-147, MMP-2, MMP-9).
  • In vitro invasion assays with and without U-0126 or siRNA-CD147.

Main Results:

  • Low-dose Ara-C did not significantly affect cell proliferation but increased invasion.
  • Ara-C upregulated MMP-2, MMP-9, CD-147 expression, and ERK activation.
  • U-0126 and siRNA-CD147 treatments inhibited Ara-C-induced invasion.

Conclusions:

  • Ara-C enhances gastric cancer cell invasiveness through CD-147/MMP-2/MMP-9 expression via the ERK signaling pathway.
  • Findings offer insights for preventing Ara-C-related collateral damage in chemotherapy.

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