Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 18, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
12:31

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

26.3K

Liposome functionalization with copper-free "click chemistry".

Erik Oude Blenke1, Gruson Klaasse2, Hannes Merten3

  • 1Department of Pharmaceutics, Utrecht Institute of Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|January 28, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapy.

EMBO molecular medicine·2026
Same author

Semi-automated Ribosome Display for High-Throughput DARPin Binder Selection.

New biotechnology·2026
Same author

Induction of SpCas9-Directed Immune Responses Using Lipid Nanoparticles and Identification of SpCas9-Derived T Cell Epitopes in C57BL/6 Mice.

The CRISPR journal·2026
Same author

Azide-functionalized SpCas9 enables generation of site-selective and bioactive Cas9-siRNA conjugates.

Chemical communications (Cambridge, England)·2026
Same author

Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state.

Journal of virology·2026
Same author

DARPins as pan-reactivators of temperature-sensitive p53 cancer mutants.

Proceedings of the National Academy of Sciences of the United States of America·2026

This study demonstrates site-specific liposome surface modification using strain-promoted azide-alkyne cycloaddition (SPAAC). This bioorthogonal click chemistry enables homogeneous conjugation of dyes and proteins, improving liposome applications in cell studies.

Area of Science:

  • Bioconjugation Chemistry
  • Liposome Technology
  • Surface Modification

Background:

  • Liposome surface modification is crucial for diverse applications.
  • Conventional conjugation methods yield heterogeneous products due to limited site control.
  • Bioorthogonal click chemistry offers a promising alternative for controlled conjugation.

Purpose of the Study:

  • To develop a site-specific method for conjugating molecules to liposomes.
  • To utilize strain-promoted azide-alkyne cycloaddition (SPAAC) for homogeneous liposome functionalization.
  • To demonstrate the conjugation of small molecules and proteins to liposomes via SPAAC.

Main Methods:

  • Preparation of cyclooctyne-functionalized lipids for liposome incorporation.
  • Conjugation of 5'-TAMRA-azide (small molecule dye) to liposomes via SPAAC.
Keywords:
Copper-free click chemistryDARPinsLiposomesSite specific conjugationStrain-promoted azido-alkyne cycloaddition

More Related Videos

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
07:23

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

8.8K
Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

13.2K

Related Experiment Videos

Last Updated: Apr 18, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
12:31

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

26.3K
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
07:23

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

8.8K
Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

13.2K
  • Conjugation of azido-DARPin (model protein) to liposomes via SPAAC.
  • Evaluation of conjugation efficiency, site specificity, and compatibility with cell studies.

    • Main Results:

    • Successful site-specific conjugation of both a small molecule dye and a model protein to liposomes was achieved using SPAAC.
    • Complete control over the number of small molecule ligands per liposome was demonstrated.
    • Sufficient protein conjugation was achieved for liposome targeting to cells.
    • The SPAAC approach avoids copper catalysts, enhancing compatibility with biological systems.

    Conclusions:

    • SPAAC provides a robust and site-specific strategy for homogeneous protein conjugation to liposomal surfaces.
    • This method eliminates the formation of dysfunctional conjugates common with traditional methods.
    • The copper-free nature of SPAAC makes it highly suitable for cell-based and in vivo applications.