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Updated: Apr 18, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
Published on: November 14, 2017
Analysis of mitochondrial DNA and RNA integrity by a real-time qPCR-based method
Wei Wang1, Ying Esbensen, Katja Scheffler
1Department of Medical Biochemistry, Institute of Clinical Medicine, Rikshospitalet, University of Oslo, Sognsvannsveien 20, 0027, Oslo, Norway.
Abstract:
This chapter describes the use of real-time qPCR to analyze the integrity of mitochondrial nucleic acids quantitatively. The method has low material requirement, is low cost, and can detect modifications with high resolution. The method is specifically designed for mitochondrial RNA and DNA, but can be easily transferred to other high-copy number cases. This procedure describes analyses of brain nucleic acids, but other tissues or cells can be analyzed similarly.

