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Diamond nanoparticles based biosensors for efficient glucose and lactate determination
M Briones1, E Casero1, M D Petit-Domínguez1
1Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Campus de Excelencia de la Universidad Autónoma de Madrid, c/Francisco Tomás y Valiente N°7, 28049 Madrid, Spain.
Biosensors & Bioelectronics
|January 31, 2015
Summary
This study modified gold electrodes with diamond nanoparticles (DNPs) to create novel electrochemical biosensors. The developed biosensor demonstrated effective lactate detection, marking a significant advancement in nanomaterial-based sensing technologies.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biosensors
Background:
- Undoped diamond nanoparticles (DNPs) possess insulating properties but exhibit surface-dependent electrochemical activity.
- Gold electrodes are versatile platforms for electrochemical applications.
- Developing novel nanomaterials for biosensing enhances sensitivity and detection capabilities.
Purpose of the Study:
- To immobilize undoped diamond nanoparticles (DNPs) onto a gold electrode for electrochemical biosensing.
- To investigate the electrochemical behavior of the DNPs/Au platform.
- To fabricate and characterize an electrochemical biosensor for lactate determination using DNPs.
Main Methods:
- Electrode modification via direct adsorption of DNPs onto a gold electrode.
- Electrochemical characterization using cyclic voltammetry (CV).
- Surface morphology analysis using atomic force microscopy (AFM) and field emission scanning electron microscopy (FE-SEM).
- Enzyme immobilization (lactate oxidase) for biosensor fabrication.
Main Results:
- DNPs on the gold electrode exhibited distinct oxidation/reduction peaks, indicating electrochemical activity due to surface functionalities.
- The DNPs/Au platform showed good electrocatalytic response for glucose detection with a redox mediator.
- A novel lactate biosensor (LOx/DNPs/Au) was successfully developed, showing a linear range of 0.05–0.7 mM, sensitivity of 4.0 µA mM⁻¹, and a detection limit of 15 µM.
Conclusions:
- Undoped diamond nanoparticles can be effectively immobilized on gold electrodes to create electrochemically active platforms.
- This work presents the first electrochemical biosensor for lactate utilizing diamond nanoparticles.
- The developed biosensor demonstrates promising performance for sensitive and selective lactate detection.
