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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
Published on: March 7, 2013
Evaluation of a mouse Y chromosome probe for assessing marrow transplantation
I N Hampson1, E Spooncer, T M Dexter
1Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, England.
Experimental Hematology
|March 1, 1989
Abstract:
The multicopy mouse Y chromosome DNA probe 80Y/B (1) has been used to probe genomic DNA isolated from male and female bone marrow cells, mixed together in known ratios. It was found that in a mixture with a ratio of 1 male:200 female marrow cells the male cells could readily be detected by this method. The potential use of this technique for assessing repopulation kinetics of marrow transplantation is discussed.
