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Contributed review: Review of integrated correlative light and electron microscopy
1Medical Cell Biophysics Group, MIRA Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
The Review of Scientific Instruments
|February 2, 2015
Summary
Correlative microscopy integrates electron and light microscopy for enhanced sample analysis. This approach combines high resolution with chemical specificity, advancing cell biology and materials science research.
Area of Science:
- Advanced microscopy techniques
- Multimodal imaging science
Background:
- Increasing demand for correlating diverse sample information (morphological, structural, chemical) in fields like cell biology and materials science.
- Limitations of individual microscopy techniques in providing comprehensive sample understanding.
Purpose of the Study:
- To introduce integrated systems combining electron microscopy and light microscopy for correlative analysis.
- To address the need for bridging resolution and contrast differences between microscopy techniques.
Main Methods:
- Development of integrated systems merging nanometer-resolution electron microscopes with optical microscopes.
- Utilizing spectroscopy for chemically or label-specific information acquisition.
- Correlating data from disparate microscopy techniques.
Main Results:
- Enables investigation of sample properties through complementary information from electron and light microscopy.
- Facilitates analysis of complex samples, such as nanometer-scale inorganic/organic material distribution.
- Supports advanced applications like live cell analysis with electron microscopy and in situ spectroscopic analysis.
Conclusions:
- Integrated correlative microscopy represents a new research field with broad applicability.
- This approach enhances understanding of sample preparation and properties.
- Future research will benefit from further development and application of these integrated systems.
