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Fourier transform two-dimensional fluorescence excitation spectrometer by using tandem Fabry-Pérot interferometer.

Hiroshi Anzai1, Neeraj Kumar Joshi2, Masanori Fuyuki3

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A novel Fourier transform two-dimensional fluorescence excitation spectrometer (FT-2DFES) was developed. This instrument effectively analyzes molecules with broad absorption bands, demonstrating separate fluorescence peaks and energy transfer in a mixed dye solution.

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Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Traditional fluorescence spectroscopy faces limitations with broad absorption bands.
  • Multiplex techniques offer advantages but require specialized instrumentation.
  • Modulating transitions in condensed-phase molecules necessitates advanced spectroscopic methods.

Purpose of the Study:

  • To develop a Fourier transform two-dimensional fluorescence excitation spectrometer (FT-2DFES).
  • To enable the analysis of molecules with large absorption bandwidths (>100 nm).
  • To demonstrate the instrument's capability in observing fluorescence excitation and energy transfer.

Main Methods:

  • Development of an FT-2DFES utilizing a tandem Fabry-Pérot interferometer (FPI) and multiplex technique.
  • Application of the tandem FPI for modulating transitions with large absorption bandwidths.
  • Analysis of a mixed methanol solution containing laser dyes (coumarin 480, rhodamine 6G, DCM, LDS750).

Main Results:

  • Successful separate observation of fluorescence excitation peaks from a mixed dye solution.
  • Demonstration of the FT-2DFES's effectiveness for condensed-phase molecules.
  • Observation of energy transfer from rhodamine 6G to LDS750.

Conclusions:

  • The developed FT-2DFES is effective for analyzing molecules with broad absorption bands.
  • The instrument enables detailed study of fluorescence excitation and energy transfer processes.
  • This technology advances spectroscopic analysis for complex molecular systems.