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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Fourier Transform Multicolor Excitation Action Spectroscopy (FT-MEAS): A Model-Free Approach for Investigating

Rinka Inoue1, Sayaka Yamashita1, Kai Fukuda1

  • 1Department of Chemistry, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Analytical Chemistry
|May 14, 2026
PubMed
Summary

We developed Fourier Transform Multicolor Excitation Action Spectroscopy (FT-MEAS), a model-free method to analyze complex photoreactions. This technique accurately quantizes multicolor light responses, advancing photosynthesis and photocatalysis research.

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Area of Science:

  • Photochemistry
  • Spectroscopy
  • Chemical Kinetics

Background:

  • Photoreactions like photosynthesis and photocatalysis involve complex, multistep processes.
  • Analyzing these reactions under photostationary conditions requires sophisticated methods.
  • Current techniques may struggle with the intricacies of multicolor excitation and multistep pathways.

Purpose of the Study:

  • To introduce a novel, model-free spectroscopic method for analyzing multicolor, multistep photoreactions.
  • To enable the quantitative estimation of partial derivatives related to multicolor excitation responses.
  • To validate the method through numerical simulations and experimental applications.

Main Methods:

  • Development of Fourier Transform Multicolor Excitation Action Spectroscopy (FT-MEAS).
  • Application of FT-MEAS to analyze photoreaction responses to multicolor excitation.
  • Estimation of first- and second-order partial derivatives and two-color cross-partial derivatives.
  • Validation using numerical simulations of a three-color, three-level system and experimental studies.

Main Results:

  • Numerical simulations showed agreement within 2% error compared to analytical results.
  • Experimental investigation of *p*-aminoazobenzene photoisomerization confirmed the method's capabilities.
  • Successfully observed and quantified first-order, second-order, and cross-term peaks.
  • Demonstrated the ability to estimate derivative values for multicolor responses.

Conclusions:

  • FT-MEAS is a valid and effective model-free method for analyzing complex photoreactions.
  • The technique accurately quantifies multicolor excitation responses.
  • FT-MEAS provides valuable insights into photochemistry, photosynthesis, and photocatalysis.