Hexamodal imaging with porphyrin-phospholipid-coated upconversion nanoparticles

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Medical Imaging

Background:

• Nanoparticles offer versatile platforms for advanced biomedical applications.
• Multimodal imaging enhances diagnostic accuracy by combining complementary information.
• Developing biocompatible imaging agents is crucial for in vivo studies.

Purpose of the Study:

• To demonstrate hexamodal imaging capabilities using novel nanoparticles.
• To create a biocompatible material by coating upconversion nanoparticles with porphyrin-phospholipids.
• To characterize the nanoparticles for various in vitro and in vivo imaging modalities.

Main Methods:

• Synthesis of upconversion nanoparticles coated with porphyrin-phospholipids.
• In vitro characterization of nanoparticle properties.
• In vivo evaluation of nanoparticle performance across multiple imaging techniques.

Main Results:

• Successful fabrication of biocompatible porphyrin-phospholipid-coated upconversion nanoparticles.
• Demonstration of simultaneous imaging using fluorescence, upconversion, PET, CT, Cerenkov luminescence, and photoacoustic tomography.
• Validation of nanoparticle efficacy in both in vitro and in vivo settings.

Conclusions:

• The developed nanoparticles enable comprehensive hexamodal biomedical imaging.
• This approach offers a promising strategy for advanced diagnostic and research applications.
• The biocompatible nature of the nanoparticles supports their translation for in vivo use.