Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

6.1K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vimentin promotes actin assembly by stabilizing ATP-actin subunits at the barbed end.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Scalable Super-Localization Optical Probing of Individual Josephson Junctions in Superconducting Devices.

Nano letters·2026
Same author

Diffusing caveolin-1 scaffolds regulate mechanosignalling.

Nature cell biology·2026
Same author

Fixed-time adaptive fault-tolerant control of a multi-mode VTOL UAV with variable prescribed performance boundaries under random disturbances.

ISA transactions·2026
Same author

Using DNA Origami to Study Nanoscale Organization of Plasma Membranes.

Nano letters·2026
Same author

From Microscale to Nanoscale Shadow Electrochemiluminescence Microscopy.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Apr 18, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.5K

Single-molecule imaging in live cell using gold nanoparticles.

Cécile Leduc1, Satyabrata Si1, Jérémie J Gautier2

  • 1Univ Bordeaux, Laboratoire Photonique Numérique et Nanosciences, Institut d'Optique & CNRS, Talence, France.

Methods in Cell Biology
|February 3, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a small, photostable gold nanoprobe for tracking proteins in live cells. This absorption-based probe overcomes limitations of fluorescence microscopy, enabling precise intracellular protein analysis.

Keywords:
BiofunctionalizationGold nanoparticlesNanobodiesPhotothermal imagingSingle-molecule tracking

More Related Videos

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.5K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

9.2K

Related Experiment Videos

Last Updated: Apr 18, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.5K
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.5K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

9.2K

Area of Science:

  • Biophysics
  • Nanotechnology
  • Cell Biology

Background:

  • Single particle tracking in live cells requires probes that are small, photostable, and do not alter molecular behavior.
  • Current fluorescence microscopy is limited by probe size, photobleaching, and blinking.
  • Tracking intracellular proteins is crucial for understanding cellular dynamics.

Purpose of the Study:

  • To synthesize and characterize a novel, small, and highly specific gold nanoprobe for live-cell protein tracking.
  • To overcome the limitations of fluorescence-based single particle tracking methods.
  • To enable long-term, high-resolution tracking of proteins in crowded cellular environments.

Main Methods:

  • Synthesis of 5-nm-diameter gold nanoparticles.
  • Functionalization of gold nanoparticles with nanobodies targeting green fluorescent protein (GFP).
  • Detection and tracking of individual gold nanoparticles using photothermal imaging microscopy in live cells.

Main Results:

  • Successfully synthesized small (5-nm) and photostable gold nanoparticles.
  • Demonstrated high-affinity targeting of GFP-tagged proteins using nanobody-functionalized probes.
  • Achieved sensitive detection and long-term tracking of individual gold nanoparticles in live cells via photothermal imaging.

Conclusions:

  • The developed gold nanoprobe offers a promising alternative to fluorescence-based probes for single particle tracking.
  • The combination of small size, photostability, specificity, and sensitive detection allows for minimal hindrance in tracking proteins within live cells.
  • This technique facilitates detailed studies of protein dynamics in confined and crowded intracellular spaces.