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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidic Single-Cell Analysis with Affinity Beads.

Michael Werner¹, Raghavendra Palankar¹, Loïc Arm¹

¹Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.

Small (Weinheim an Der Bergstrasse, Germany)

|February 3, 2015

Summary

This summary is machine-generated.

Researchers developed a novel method using affinity beads to capture intracellular analytes. This technique involves phagocytosis, photochemical release, optical trapping, and cell lysis for sample analysis.

Keywords:

affinity beads microfluidics optical tweezers photochemical internalization single-cell analysis

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

Phagocytosis is a key cellular process for internalizing particles.
Analyzing intracellular analytes is crucial for understanding cellular functions.
Current methods for isolating intracellular analytes can be complex and inefficient.

Purpose of the Study:

To develop a novel method for capturing and isolating intracellular analytes.
To utilize affinity beads for targeted intracellular analyte retrieval.
To enable subsequent analysis of captured intracellular components.

Main Methods:

Micrometer-sized affinity beads were introduced to cells.
Beads were internalized via phagocytosis.
Photochemical methods were used to release beads from phagosomes.
Optical trapping was employed for bead manipulation within the cell.
Cell lysis facilitated the isolation of beads and captured analytes.

Main Results:

Successful uptake and release of affinity beads from phagosomes.
Demonstrated ability to optically trap beads within the cell.
Efficient isolation of intracellular analytes captured by the beads.
The method allows for subsequent analysis of the isolated analyte.

Conclusions:

This novel technique provides an effective strategy for isolating intracellular analytes.
The combination of phagocytosis, photochemical release, and optical trapping offers a versatile tool for cell biology research.
This method facilitates the analysis of previously inaccessible intracellular targets.