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Protein Dynamics in Living Cells

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Updated: Apr 17, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

PAD2 Activity Monitored via a Fluorescent Substrate Analog.

Mary J Sabulski¹, Yanming Wang², Marcos M Pires¹

¹Chemistry Department, Lehigh University, Bethlehem, PA, 18015, USA.

Chemical Biology & Drug Design

|February 4, 2015

Summary

We developed a novel profluorescent assay to monitor peptidyl arginine deiminase 2 (PAD2) activity and inhibition. This tool aids in understanding PAD2

Keywords:

PAD2 PAD4 citrullination fluorescence assay inhibitor

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Last Updated: Apr 17, 2026

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Area of Science:

Biochemistry
Enzymology
Chemical Biology

Background:

Peptidyl arginine deiminase 2 (PAD2) catalyzes the conversion of arginine to citrulline, a post-translational modification impacting protein properties.
Dysregulated PAD2 activity is linked to various human diseases, highlighting its potential as a therapeutic target.
Small molecule inhibitors of PAD2 are crucial for probing its role in disease pathogenesis.

Purpose of the Study:

To develop a robust assay for monitoring PAD2 activity.
To create a tool for screening PAD2 inhibitors.
To facilitate drug discovery efforts targeting PAD2.

Main Methods:

Design and synthesis of a profluorescent substrate analog for PAD2.
Establishment of a robust assay to detect PAD2 activity and inhibition.
Characterization of the assay's performance and utility.

Main Results:

A novel profluorescent substrate analog was successfully developed.
The assay effectively reports on PAD2 activity.
The assay demonstrates utility in monitoring PAD2 inhibition by small molecules.

Conclusions:

The developed profluorescent assay provides a valuable tool for studying PAD2.
This assay is suitable for drug discovery efforts aimed at inhibiting PAD2.
Future research can leverage this assay to explore PAD2's role in disease and develop new therapeutics.