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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Apr 17, 2026

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Applying superresolution localization-based microscopy to neurons.

Haining Zhong¹

¹Vollum Institute, Oregon Health & Science University, Portland, Oregon, 97239; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, 20147.

Synapse (New York, N.Y.)

|February 5, 2015

Summary

This summary is machine-generated.

Superresolution microscopy, including photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), enables nanoscale visualization of brain structures. This technique is crucial for understanding neuronal organization and function.

Keywords:

LBM PALM/STORM PSD-95 light microscopy/EM image correlation superresolution imaging

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Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Related Experiment Videos

Last Updated: Apr 17, 2026

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Area of Science:

Neuroscience
Cell Biology
Microscopy

Background:

Precise protein and signaling molecule localization is essential for brain function.
Conventional light microscopy cannot resolve structures at the nanometer scale required for studying molecular organization.
Superresolution microscopy offers a solution to visualize nanoscale cellular architectures.

Purpose of the Study:

To discuss the principles and applications of superresolution, localization-based fluorescent microscopy (LBM) in neuroscience.
To highlight the potential of LBM in revealing neuronal organization.
To present a new sample preparation procedure for applying LBM to brain tissue.

Main Methods:

Localization-based fluorescent microscopy (LBM) techniques, including photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM).
Development and testing of a novel sample preparation procedure for brain tissue.
Analysis of nanoscale cellular architectures and molecular organization in neurons.

Main Results:

LBM provides a resolving power at the 10 nm scale, surpassing conventional microscopy limitations.
LBM has already revealed previously unknown cellular architectures and organizational principles in neurons.
The study presents unpublished experimental results for a new LBM sample preparation protocol.

Conclusions:

Superresolution LBM is a vital emerging tool for modern neuroscience research.
LBM has the potential to significantly advance our understanding of neuronal function and organization.
Further development of sample preparation techniques is necessary to fully realize the potential of LBM in studying brain tissue.