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Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is

Stephen Chu-Sung Hu1, Chi-Ling Lin2, Hui-Min Cheng3

  • 1Department of Dermatology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan ; Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Evidence-Based Complementary and Alternative Medicine : Ecam
|February 5, 2015
PubMed

Insights

Artocarpin, a natural compound, effectively reduced human squamous cell carcinoma (SCC) viability and induced apoptosis. Its efficacy was higher in low protein concentrations, suggesting potential for SCC treatment.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Oncology

Background:

  • Artocarpin is a prenylated flavonoid with known biological activities.
  • The effects of artocarpin on human cutaneous squamous cell carcinoma (SCC) remain uninvestigated.

Purpose of the Study:

  • To determine the cytotoxic effects of artocarpin on human cutaneous SCC cells.
  • To investigate the dependence of artocarpin's pharmacological activity on protein-nutrient concentration.

Main Methods:

  • Treatment of HSC-1 cells (human cutaneous SCC cell line) with artocarpin.
  • Assessment of cell viability, apoptosis (caspase 3/7 activity), and protein signaling pathways (MAPK, Akt/mTOR).
  • High-performance liquid chromatography (HPLC) analysis to determine artocarpin uptake under varying fetal bovine serum (FBS) concentrations.

Main Results:

  • Artocarpin decreased HSC-1 cell viability and induced apoptosis.
  • Effects were more pronounced at low FBS concentrations.
  • Artocarpin modulated MAPK and Akt/mTOR signaling pathways.
  • High FBS concentrations inhibited artocarpin uptake into cells.

Conclusions:

  • Artocarpin induces apoptosis in human cutaneous SCC cells via MAPK and Akt/mTOR pathway modulation.
  • Protein binding in FBS may reduce artocarpin's cellular uptake and cytotoxic activity.
  • Artocarpin shows potential as a future therapeutic agent for cutaneous SCC.

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