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Updated: Apr 17, 2026
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Published on: May 28, 2017
Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral
Andrielle Castilho-Fernandes1, Aparecida Maria Fontes, Kuruvilla Joseph Abraham
1Faculty of Medicine of Ribeirão Preto, Center for Cell Therapy and Regional Blood Center, University of São Paulo, Ribeirão Preto, Brazil.
Abstract:
Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive elements, fragile sites and target genes. In HepG2, rhFIX was had an increased preference for chromosomes 6 and 17; the median distance to the nearest CpG islands was 15,240 base pairs and 37 % of the integrations occurred in RefSeq genes. In Hek293, rhFIX had an increased preference for chromosome 5; the median distance to the nearest CpG islands was 209,100 base pairs and 74 % of the integrations occurred in RefSeq genes. The integrations in both cell lines were distant from the TSS. The integration patterns associated with this vector are different in each cell line.
