Effects of ethanol on the expression of caveolin-1 in HepG2 cells

This study aimed to investigate the effects of ethanol on the expression of caveolin‑1 (CAV‑1) in HepG2 hepatocarcinoma cells. Ethanol‑treated HepG2 cells were investigated using the in vitro model to determine whether ethanol can influence the expression of CAV‑1. Cell viability was measured using the colorimetric 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Expression of CAV‑1 was detected using western blot analysis. Quantitative PCR (qPCR) was used to determine CAV‑1 mRNA levels. The distribution of CAV‑1 in HepG2 cells was analyzed using immunofluorescence. The MTT assay results revealed that cell viability was not altered at ethanol concentrations of <1.0%, while ethanol concentrations >1.0% caused cell shedding, but not cell fragmentation. Western blot analysis showed significant differences in the levels of CAV‑1 expression between the control group and the 1.0% ethanol‑treated group at 6, 12 and 24 h (all P<0.05). qPCR showed significant differences in the expression levels of caveolin‑1 mRNA between the control group and the 1.0% ethanol‑treated group at 6 h, 12 h and 24 h (all P<0.05). Immunofluorescence demonstrated that CAV‑1 was distributed discontinuously at the boundaries of HepG2 cells. The results indicate that ethanol may increase the expression of CAV‑1 in HepG2 cells.