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Updated: Apr 17, 2026
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Stromal bed thickness measurement during laser in situ keratomileusis using intraoperative optical coherence
Cong Ye1, Marco Yu, Vishal Jhanji
1*Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China; †Department of Mathematics and Statistics, Hang Seng Management College, Hong Kong SAR, China; and ‡Centre for Eye Research Australia, University of Melbourne, Victoria, Australia.
Purpose:
The aim of this study was to compare repeatability and agreement of stromal bed thickness (SBT) measurements with intraoperative spectral domain optical coherence tomography (SD-OCT) and ultrasound pachymetry (USP).
Methods:
The SBT was measured in 14 eyes of 14 patients during femtosecond laser in situ keratomileusis (LASIK). Intraoperative SD-OCT was positioned on the eye to acquire images after creation of LASIK flaps. Patients were then asked to sit in front of a swept-source optical coherence tomography (SS-OCT) for measurements. Subsequently, LASIK flaps were lifted and SBT was measured with USP. Three readings were obtained with each instrument. Repeatability of measurements was evaluated by the intraclass correlation coefficient (ICC) and reproducibility coefficient (RC).
Results:
ICC and RC values of SBT measurements were 0.98 and 8.7 μm, 0.99 and 7.1 μm, and 0.99 and 6.9 μm, respectively, for SD-OCT, SS-OCT, and USP. There were no significant differences in the RC between the devices. The ICCs for agreement of SBT measurement were 0.97 between SD-OCT and SS-OCT, 0.8 between SD-OCT and USP, and 0.8 between SS-OCT and USP. The 95% limits of agreement of SBT measurements were -13.1 to 8.8 μm between SD-OCT and SS-OCT, -48.7 to 31.4 μm between SD-OCT and USP, and -42.8 to 29.8 μm between SS-OCT and USP.
Conclusions:
SBT measurements obtained with intraoperative SD-OCT, SS-OCT, and USP were highly repeatable. Optical coherence tomography may be used as a potential noncontact device for corneal thickness assessment during LASIK. However, optical coherence tomography tends to underestimate the SBT compared with USP, and these measurements may not be used interchangeably.
