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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Activity-based protein profiling: recent advances in probe development and applications.

Pengyu Yang1, Kai Liu

  • 1Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037 (USA).

Chembiochem : a European Journal of Chemical Biology
|February 6, 2015
PubMed
Summary
This summary is machine-generated.

Activity-based protein profiling (ABPP) decodes the function of proteins from genomic data. This method uses small molecules to identify protein targets, aiding biochemical analysis and drug discovery.

Keywords:
activity-based protein profilingbioorthogonalchemical proteomicsclick chemistrytandem labeling

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Area of Science:

  • Genomics
  • Proteomics
  • Chemical Biology

Background:

  • The human genome project revealed ~20,000 genes, but protein functions remain largely unknown.
  • Translating genomic information into cellular functions presents a significant scientific challenge.
  • Activity-based protein profiling (ABPP) offers a complementary approach to gene expression analysis.

Purpose of the Study:

  • To introduce recent advances in Activity-based Protein Profiling (ABPP).
  • To highlight the applications of ABPP in decoding genomic information and understanding protein function.
  • To showcase ABPP as a tool for rapid biochemical analysis and inhibitor discovery.

Main Methods:

  • ABPP utilizes synthetic small molecules to covalently label active proteins.
  • These labeled proteins are then identified using various biochemical and proteomic techniques.
  • The approach enables the analysis of protein function in a cellular context.

Main Results:

  • ABPP facilitates the identification of protein targets and their functions.
  • It enables rapid biochemical analysis, complementing traditional methods.
  • The technique aids in the discovery of novel inhibitors for therapeutic targets.

Conclusions:

  • ABPP is a powerful and versatile tool for understanding the proteome.
  • It bridges the gap between genomic information and functional protein insights.
  • ABPP is crucial for advancing biochemical analysis and drug discovery efforts.